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The immunodiffusion test has been routinely used to detect bee viruses because it is rapid, inexpensive and specific [1]. This technique, however, is relatively insensitive and the samples must contain sufficient virus to be readily detected. Stoltz et al. [4] used reverse transcription-polymerase chain reaction (RT-PCR) to detect Kashmir bee virus (KBV). This technique requires the time-consuming virus purification and RNA extraction steps. Direct RT-PCR can circumvent these steps [2, 3]. Here we report a protocol that further simplifies the use of RTPCR in bee virus detection.

A scientific note on the detection of Kashmir bee virus in individual honeybees and Varroa jacobsoni mites