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Semen from the honey bee, Apis melfifera, was stored for 113 days in three different treatments : a) rapid cool, b) slow cool, and c) over N2 gas. The aged semen was added to equal parts of fresh semen and used for insemination of virgin queen bees. Progeny from eggs fertilized by stored semen could be distinguished from that of fresh semen by a genetic marker. The storage of bee semen under N2, at 13 °C and in tubes dusted with streptomycin sulfate, yielded greater fertility after insemination than has previously been reported. Four progeny samples collected at least 2-4 weeks apart showed increasing proportions of progeny from the stored, aged, and senescent semen. We conclude that the mixture of fresh semen with aged

ENHANCED FERTILITY OF HONEY BEE SEMEN STORED IN VITRO AND POSSIBLY A REVERSAL OF SENESCENCE