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International audienceA nanosecond time resolved fluorescence, derived from the nucleus Raphe dorsalis (nRD) of the rat is reported. A single optical fiber is used for the transmission of the subnanosecond nitrogen laser pulse (337nm) and the collection of the NADH brain fluorescence at 460nm. This method also combined with polygraphic recordings, is used in animals under long-term chronic conditions and unanesthetized. Thoughout the Sleep-Wake cycle and versus the waking state (W), the NADH peak measured increases during slow wave sleep (SWS) and paradoxical sleep (PS) or during PS only. The variations observed are dependent upon the optical fiber location within the nRD. The existence of oxidation mechanisms for sleep triggering and maintenance are discussed

Fluorescence induite par laser sur les tissus profonds du cerveau du rat : application à l'étude du cycle veille-sommeil