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Latin square design. They were fed a diet (7 kg DM) consisting of hay with 0 (BO), 30 (B30) or 60 % (B60) barley. Two cereals, rapidly (barley) and slowly (corn) degradable, ground through a 4 mm-screen, were put in polyester bags and introduced in the rumen at feeding time on two consecutive days. Bags and rumen digesta were with drawn 2 h or 23 h after feeding. Enzymes of solid adherent microorganisms, the greatest microbial population in term of biomass (Craig et al, 1987, J Nutr, 117, 56-62) and amylolytic activity (Martin et al, 1993, Curr Microbiol, 27, 223-228), were extracted by freezingdefrosting and sonication under anaerobic conditions (Noziere and Michalet-Doreau, 1994, Reprod Nutr Develop, 34, 281-288). They were incubated with starch at 39°C. Amylase activity was expressed as pmol glucose released per mg protein for 1 h. Amylolytic activity extracted from rumen content was maximum 2 h after feeding. This activity increased from 1.1 to 9.7 units respectively with 0 and 60 % barley in the diet. Amylolytic enzyme capacity would not limit ruminal starch digestion since no plateau in the amylolytic activity was observed with increased level of starch in the diet.

Amylolytic activity of solid-adherent microorganisms in rumen content and bag residues: influence of diet and nature of cereals into bags