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Two-dimensional (2D) correlation analysis has been applied to analyze protein and fat concentration-dependent near-infrared (NIR) spectral variations of milk. Synchronous and asynchronous 2D correlation spectra of milk enhance spectral resolution and provide information about concentration-dependent intensity changes not readily accessible from one-dimensional spectra. The asynchronous 2D correlation map shows marked differences between the protein and fat concentration-dependent spectral changes.

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Potential of two-dimensional correlation spectroscopy in analyses of NIR spectra of biological fluids. I. Two-dimensional correlation analysis of protein and fat concentration-dependent spectral variations of milk