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Summary — An immunogold labelling procedure was developed to detect pea seed-borne mosaic virus (PSbMV) virion and pinwheel inclusion body proteins in leaf and seed tissues of pea and to study the cytology of PSbMV infection in this host. Ultrathin sections were treated with immunoglobulin prepared against either PSbMV particles or PSbMV pinwheel inclusion bodies. The sections were then probed with gold-labelled goat antirabbit immunoglobulin. With immunoglobulin against PSbMV particles, virus particles, but not pinwheel and crystalline inclusions, were specifically labelled. With immunoglobulin against pinwheel inclusion bodies, pinwheel inclusion bodies (bundles and scrolls) were specifically labelled, but neither PSbMV particles nor its crystalline inclusion bodies were labelled, showing that the crystalline inclusion body protein was unrelated to either virus coat protein or the pinwheel inclusion protein. When cotyledon and plumule sections cut from fixed seed tissues were treated with the 2 types of immunoglobulin, only PSbMV particle bundles were labelled, indicating that in seed tissues, the pinwheel inclusion was structurally different from that in leaf tissues.

agronomie

Detection of pea seed-borne mosaic virus virion and pinwheel inclusion body proteins in leaf and seed tissues of pea (Pisum sativum L) by immunogold labelling