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We present the results of a search for the negative ion C4H- in various darkclouds, low mass star-forming regions and photon-dominated regions (PDRs). Wehave also searched for C6H-, C2H- and CN- in some of the sources. Themillimeter-wave observations were carried out with the IRAM-30m telescope. Wedetect C4H-, through the J = 9-8 and J = 10-9 rotational transitions, in thelow mass star-forming region L1527. We thus confirm the tentative detection ofthe J = 9-8 line recently reported toward this source. The [C4H-]/[C4H] ratiofound is 0.011 %, which is slightly lower than the value observed in IRC+10216, 0.024 %, but above the 3 sigma upper limit we derive in TMC-1, < 0.0052%. We have also derived an upper limit for the [C6H-]/[C6H] ratio in theHorsehead Nebula, and for various anion-to-neutral ratios in the observedsources. These results are compared with recent chemical models.

Search for anions in molecular sources: C4H- detection in L1527