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Multinuclear n.m.r. spectroscopy, including the use of two-dimensional methodology, was used to confirm the identity of inositol 1,3,4-triphosphate and its metabolic precursor inositol 1,3,4,5-tetrakisphosphate. The cyclohexane ring in each molecule exhibits a chair conformation with all phosphate groups occupying equatorial positions.

biochemical journal

Confirmation of the identities of inositol 1,3,4-trisphosphate and inositol 1,3,4,5-tetrakisphosphate by the use of one-dimensional and two-dimensional n.m.r. spectroscopy