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The membranes of intact C6 cells were enriched with phosphatidyldimethylethanolamine or phosphatidylmonomethylethanolamine. These cells showed enhanced rates of phospholipid methylation but this was not accompanied by an increased beta-adrenergic response. We conclude that phospholipid methylation is not coupled to the activation of adenylate cyclase in the beta-adrenergic response of C6 glial cells.

The effect of polar head group substitution on phospholipid methylation and the β-adrenergic response in C6 glial cells