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1-(N-Amino-n-hexyl)carbamoylimidazole hydrochloride was synthesized and shown to be a potent irreversible inhibitor of human urokinase (EC 3.4.21.31), pig kidney-cell plasminogen activator (EC 3.4.21.-), human plasmin (EC 3.4.21.7) and bovine pancreatic beta-trypsin (EC 3.4.21.4). The kinetics of inhibition of the enzymes were determined by monitoring the hydrolysis of an appropriate fluorogenic substrate. Bovine thrombin and Factor Xa are hardly affected by the inhibitor.

biochemical journal

The irreversible inhibition of urokinase, kidney-cell plasminogen activator, plasmin and <i>β</i>-trypsin by 1-(<i>N</i>-6-amino-n-hexyl)carbamoylimidazole

The irreversible inhibition of urokinase, kidney-cell plasminogen activator, plasmin and β-trypsin by 1-(N-6-amino-n-hexyl)carbamoylimidazole