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Nitrobenzylthioninosine, a potent nucleoside-transport inhibitor, binds specifically to functional nucleoside transport sites. Irradiation of freeze-dried human erythrocyte membranes with high-energy electrons was used to estimate the apparent molecular weight of the nitrobenzylthioninosine-binding complex in situ. The nitrobenzylthioinosine-binding complex had an apparent mol.wt. of 122000.

biochemical journal. cellular aspects

Nucleoside transport in human erythrocytes. Apparent molecular weight of the nitrobenzylthioinosine-binding complex estimated by radiation-inactivation analysis