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The temperature-dependence of the ribonuclease A-catalysed hydrolysis of cytidine 2':3'-phosphate was studied in the range of temperatures 0--40 degrees C. A break at 4 degrees C was found both in the Arrhenius and the van't Hoff plots. It is likely that the transition observed is due to the change in the structure of water.

biochemical journal

Thermodynamic studies on the hydrolysis of cytidine 2′:3′-phosphate by bovine pancreatic ribonuclease A. A possible effect of the change of the structure of water