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Descriptions are given for the graphical determination of molecular pK values from the curves of logV and p(Km/V) against pH. The arguments are based on the model dissociation equilibria LAL in equilibrium (AL + LA) in equilibrium A, where A denotes a molecule having two binding sites for a ligand L.

Graphical determination of pK values of the active-site groups of enzymes. An analysis of the bell-shaped curves