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The isolated perfused rat liver system has been used to monitor the utilization of N-[3H]acetyl-D-galactosamine and N-acetyl-D-[1-14C]galactosamine for the biosynthesis of radiolabelled glycoproteins, which are subsequently secreted into the plasma. Both radiolabels appear in a number of different glycoproteins, predominantly as sialic acid and N-acetylglucosamine. The ratio of labelled sialic acid to labelled N-acetylglucosamine varies for different glycoproteins, but the bulk of N-acetyl-D-galactosamine is incorporated without deacetylation.

biochemical journal. cellular aspects

Utilization by the isolated perfused rat liver of <i>N</i>-acetyl-<scp>d</scp>-[1-14C]galactosamine and <i>N</i>-[3H]acetyl-<scp>d</scp>-galactosamine for the biosynthesis of glycoproteins

Utilization by the isolated perfused rat liver of N-acetyl-d-[1-14C]galactosamine and N-[3H]acetyl-d-galactosamine for the biosynthesis of glycoproteins