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The efficacy of class-I and class-II aldolases in catalysing the C-1 proton exchange in fructose 1,6-bisphosphate and dihydroxyacetone phosphate was investigated. The rate of this reaction was at least two orders of magnitude slower in class-II than in the class-I aldolases. It is suggested that this difference reflects the formation of different intermediates in the reactions catalysed by the two classes of aldolase.

biochemical journal

The proton exchange of the <i>pro</i>-<i>S</i> hydrogen atom at C-1 in dihydroxyacetone phosphate and <scp>d</scp>-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases

The proton exchange of the pro-S hydrogen atom at C-1 in dihydroxyacetone phosphate and d-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases