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1. By using an optical method the kinetic parameters of hexose transport across the human erythrocyte membrane were determined for several sugars. The series of half-saturated constants is as follows: 3-deoxy-3-fluoro-d-glucose = 3-O-methyl-d-glucose &lt;d-glucose&lt;d-mannose &lt;3-deoxy-d-glucose&lt;d-galactose&lt;l-arabinose. 2. Estimations of the dissociation energy of the 3-deoxy-3-fluoro-d-glucose-carrier and d-glucose-carrier complexes suggest that the binding of the two sugars to the transport system is equivalent. 3. Incubation of the erythrocytes with 3-deoxy-3-fluoro-d-glucose results in a small but significant release of F(-) anion. Cells treated in this way lose their ability to transport glucose.

The interaction of 3-deoxy-3-fluoro-d-glucose with the hexose-transport system of the human erythrocyte