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Four subfractions of histone F1 from rat thymus were obtained by preparative polyacrylamide-gel electrophoresis. These subfractions are closely related in primary structure but show marked differences in radioactivity when derived from rat thymus nuclei labelled in vitro with [(32)P]phosphate.

Histone F1 from rat thymus. Subfractionation and incorporation of [32P]phosphate in vitro