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1. The effect of administration of various dosages of dimethylnitrosamine on the extent of methylation of liver and kidney nucleic acids in the intact rat was studied. Methylation of liver nucleic acids was linearly related to the dosage, but decreasing the dose produced relatively less lowering of the extent of alkylation of kidney nucleic acids. 2. The rates of disappearance of 7-methylguanine from DNA during the 2 days after administration of dimethylnitrosamine in the intact animal and on incubation under simulated physiological conditions in vitro were compared. At a high dosage this rate was greater in vivo than in vitro. At a low dosage the small difference between the two rates was not thought to be sufficient evidence for existence of a specific enzymic excision of the abnormal base.

biochemical journal. cellular aspects

Stability of deoxyribonucleic acid methylated in the intact animal by administration of dimethylnitrosamine. Rate of breakdown <i>in vivo</i> and <i>in vitro</i> at different dosages

Stability of deoxyribonucleic acid methylated in the intact animal by administration of dimethylnitrosamine. Rate of breakdown in vivo and in vitro at different dosages