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1. Nitrogen-fixing preparations from Azotobacter chroococcum reduced substrates with the following K(m) values: methyl isocyanide, 1.8x10(-4)m; ethyl isocyanide, 2.5x10(-2)m; cyanide ion, 1.4x10(-3)m; acetylene, 1.2x10(-4)m. 2. Nitrogen, carbon monoxide or hydrogen competitively inhibited isocyanide reduction with the following K(i) values: hydrogen, 1.3x10(-3)m; carbon monoxide, 6.8x10(-6)m; nitrogen, 4.3x10(-4)m. 3. Living nitrogen-fixing bacteria, and isolated clover nodules, formed methane from methyl isocyanide. 4. These results are discussed in relation to other work and possible mechanisms of nitrogen fixation.

biochemical journal. cellular aspects

The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from <i>Azotobacter chroococcum</i> and the effects of inhibitors

The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from Azotobacter chroococcum and the effects of inhibitors