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A sensitive method using the Technicon Amino Acid Autoanalyzer was developed for the determination of l-tyrosine O-sulphate in mammalian urine. Normal rabbit urine was shown to contain a material that has properties identical with l-tyrosine O-sulphate. The material is also a normal constituent of rat, sheep, calf and mouse urine and is excreted by these animals in quantities ranging from 0.1 to 0.8mg./day/kg. body wt.

The detection and determination of l-tyrosine O-sulphate in rabbit and other mammalian urine