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Teichoid acids are widely distributed in the walls of lactobacilli (Baddiley & Davison, 1961; Ikawa & Snell, 1960) and the intracellular variety has been found in all members of the genus so far examined. The serological importance of these compounds as group precipitinogens is now well established (Baddiley & Davison, 1961; Sharpe, Davison & Baddiley, 1964), and the detailed chemical structure of individuals is significant in efforts to relate structure with immunological specificity. The teichoic acid from the walls ofLactobacillus buchneri N.C.I.B. 8007 is particularly interesting. This organism is in serological group E, and it has been shown that the group precipitinogen in this case is the wall teichoic acid (Sharpe et al. 1964); conrequently, it would be valuable to compare structural features, especially the nature and configuration of the sugar residues, with those of group E precipitinogens from other members of the group. Detailed chemical knowledge of the teichoic acid from the walls of this organism could also be valuable in efforts to establish the mechanism of biosynthesis of these polymers. L. buchneri would appear to be particularly suitable for such studies as it grows well in the laboratory, its walls are easily prepared in clean condition and they contain substantial amounts of teichoic acid. Moreover, as the polymer contains glycerol and glucose, isotopically labelled nucleotide precursors are reasonably available. The simple chemical degradations described below would ensure the unambiguous characterization of the teichoic acid formed in enzyme experiments.

The teichoic acid from the walls of Lactobacillus buchneri N.C.I.B. 8007