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4. Particle-size distribution data for Hevea latex suggest that in vivo the rate of initiation of new rubber particles in the aqueous or other non-rubber phase of latex may be very slow, compared with the rate of incorporation of isopentenyl pyrophosphate into rubber at the surface of existing particles. Experimentally, the conversion of isopentenyl pyrophosphate into rubber in the aqueous phase of latex in vitro is so small that it could be accounted for by incomplete removal of rubber during centrifugal separation of the aqueous phase. 5. Hevea bra8iliensi8 latex contains a proportion of non-rubber particles bounded by an osmotically sensitive membrane (lutoid particles) which, on disruption, hydrolyse ATP and thereby retard the incorporation of mevalonate into rubber. Disrupted lutoids also hydrolyse isopentenyl pyrophosphate to isopentenol, but possess no detectable isopentenyl pyrophosphate-isomerase activity. Lutoid particles are not necessary for the incorporation of isopentenyl pyrophosphate into rubber in vitro. This work forms part of the research programme undertaken by the Natural Rubber Producers' Research Association. The authors thank Dr E. D. C. Baptiste and Mr C. A. de Silva of the Rubber Research Institute of Ceylon for their generous help in providing many of the latex samples.

THE ISOLATION, CHARACTERIZATION AND ACID-BASE PROPERTIES OF RIBONUCLEIC ACID FROM RABBIT-RETICULOCYTE RIBOSOMES