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Prothidium caused gross loss of motility and clumping of all trypanosomes. Comparison of the findings for trypanosomes with those for other cells previously studied shows that T. Iewi8i iS very similar electrophoretically to freely circulating cells such as lymphocytes and tumour cells. On the other hand, the surface properties of the blood-stream form of T. rhodesiense are different electrophoretically from any other freely circulating cell so far studied. If the surface of these cells does contain carboxyl groups, the previous hypothesis that all cells carrying surface carboxyl groups will be adhesive to phagocytes will need modification (Bangham & Pethica, 1960). The absence of net charge on the freelycirculating blood-stream form of T. rhodesiense is evidence that van der Waals forces play a very minor role in cell adhesion (Pethica, 1961), although the flagellate motility may adversely influence the adhesion process.

FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES