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cells, which makes it abundantly clear that the Tween dispersions also are not absorbed without prior hydrolysis. We have shown here (Tables 2 and 3) that the major portion of the vitamin A alcohol of the mucosal cell is invariably found associated with the particulate materials. Similar observations were made with the intestinal mucosa of the rat (Ganguly et al. 1959) and the guinea pig (Glover, Green & Stainer, 1959). The presence of small amounts of the fed ester in these particles might mean that it is actually adsorbed on the outer surface of the mucosal-cell membrane and is in the process of being hydrolysed. At the same time, in every case, there was some palmitate also in the particulate fraction. It is possible that it represents the ester that is being resynthesized on the particles after the free alcohol has crossed the cell membrane; the larger concentration of the palmitate in the supernatant fraction would suggest that once resynthesized it is automatically released into the cytoplasm. SUMMARY

STUDIES ON METABOLISM OF VITAMIN A. 4. STUDIES ON THE MODE OF ABSORPTION OF VITAMIN A BY RAT INTESTINE IN VITRO