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In a preliminary communication (Trudinger, 1958) the separation of some soluble cytochromes of the autotrophic sulphur bacterium Thiobacillue X was briefly reported. Addition of thiosulphate to intact cells results in the rapid reduction of the cytochromes and the present work was undertaken to attempt to characterize the cytochromes and to obtain some information on the electron-transport chain involved in the oxidation of thio-

Thiosulphate oxidation and cytochromes in Thiobacillus X. 1. Fractionation of bacterial extracts and properties of cytochromes