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The synthesis of antibody in vitro, in common with other protein synthesis, can be inhibited by the addition ofamino acid analogues to the medium (Stavitsky & Wolf, 1958; Vaughan, Dutton, Dutton George & Marston, 1960). In an earlier paper it was shown that antibody synthesis could also be inhibited by the purine antagonist 8-azaguanine (Dutton, Dutton & George, 1958). It was established in a spleen-cell system, however, that 8azaguanine, in addition to inhibiting the synthesis of ribonucleic acid, was affecting other pathways of metabolism and was thus of little use in elucidating biochemical pathways involved in the formation of antibody (Dutton et al. 1958). The present paper describes the inhibition of antibody formation by the thymidine analogue 5-bromouracil deoxyriboside, which may be expected to affect a much more limited number of metabolic pathways. The incorporation of amino acids into the trichloroacetic acid-insoluble fraction of the spleen cells is not affected. The inhibitor appears to affect deoxyribonucleic acid but not ribonucleic acid metabolism. The implications of these findings are discussed. A preliminary account of part of this work has already been presented (Dutton, Dutton & Vaughan, 1959a, b).

The effect of 5-bromouracil deoxyriboside on the synthesis of antibody in vitro