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Studies on the distribution of solutes have shown that isolated rat-liver mitochondria are partially permeable to sucrose, sodium chloride and potassium chloride (Amoore & Bartley, 1958). In the work to be described, similar methods were employed to determine the permeability of mitochondria to certain anions of metabolic importance. Pyruvate, succinate, citrate, phosphate, adenosine 5'-phosphate and adenosine triphosphate were chosen, because there is presumptive evidence from the intracellular distribution of enzymes that in the course of metabolism these solutes probably traverse the mitochondrial membrane. For example, pyruvate formed by glycolysis in the soluble fraction of liver cells is destroyed by oxidation in the mitochondria (Siekevitz, 1957). Furthermore, there are indications from earlier work that the membrane may impose a permeability barrier upon the movements of these solutes. Thus Schneider, Striebich & Hogeboom (1956) showed that isolated mitochondria could retain their endogenous citrate if their structure was intact. The distributions of solutes were measured at 00 to minimize the transformation of metabolites or active transport by the mitochondria. The distributions were determined at three external concentrations of the anion: mm, 10 mM and in the range 32-92 mm. The lowest concentration was intended to reveal any adsorption of the anion by the mitochondria; the middle concentration was taken as representative of those often employed in metabolic experiments in vitro; and the highest concentration was designed to measure the permeability of the mitochondria when any contribution by adsorption could be proportionately small.

biochemical journal. cellular aspects

The permeability of isolated rat-liver mitochondria at 0° to the metabolites pyruvate, succinate, citrate, phosphate, adenosine 5′-phosphate and adenosine triphosphate