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Hyperactivity of the adrenal cortex in human subjects due to malignant neoplasm, hyperplasia or stimulation by adrenocorticotropic hormone (ACTH) has frequently been observed to be associated with the urinary excretion of considerable amounts of various C21 20-methylsteroids (see Table 1). possibility and because of the widespread therapeutic use of 17-hydroxy-1 1-dehydrocorticosterone (cortisone), it was thought that a specific method for the quantitative determination in urine of Cs, 17:20-dihydroxy-20-methylsteroids which might be formed from these two hormones (and from 17hydroxy-1 1-deoxycorticosterone) by metabolic re-

A method for the quantitative determination in urinary extracts of C21 17:20-dihydroxy-20-methylsteroids