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1. Sulphapyridine containing 35S has been synthesized. 2. Metabolic experiments with this compound in the rabbit have shown that there is no complete catabolism to inorganic sulphate and no significant retention of the drug in the tissues after 3 days. 3. Differences between the values obtained by assays of 3S and diazotizable material suggested the formation of a metabolite which does not diazotize or couple normally but this has not yet been isolated. We wish to express our sincere gratitude to Prof. A. Wormall for his advice and encouragement throughout this work as well as for providing facilities in his laboratory for the radioactivity counts. We had the benefit of discussing preliminary plans with Dr J. C. Boursnell. We also wish to thank our Birmingham colleagues Prof. M. L. Oliphant, F.R.S., Prof. P. B. Moon, F.R.S., and the late Dr F. E. Whitmore for their interest and advice. The 35S was obtained from the U.S. Atomic Energy Control through the kind cooperation of Dr A. S. McFarlane and the Medical Research Council. The diazo estimations were carried out by Mr P. B. Wood. Part of the expenses of the work were defrayed by a Government Grant of the Royal Society to whom grateful acknowledgement is made.

biochemical journal. cellular aspects

The fate of certain organic acids and amides in the rabbit. 10. The application of paper chromatography to metabolic studies of hydroxybenzoic acids and amides