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THE development of a technique for the satisfactory milking of small laboratory animals [Cox & Mueller, 1937; Temple & Kon, 1937] and the recent progress in the chemical and physical estimation of various vitamins have made it possible to measure several of these factors in the milk of rats and of guinea-pigs. We have examined the milks of both species for vitamin A and carotene, vitamin B1, riboflavin and vitamin C. We have also studied the effect of changes in the diet on the levels of these factors in rat's milk.

Vitamins in rat's and in guinea-pig's milk