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IN all earlier paper [Weil-Malherbe, 1938] the formation of a reducing substance from acetoacetic acid by rat kidney slices was reported. It will be shown that this substance is very probably glucose. This observation is of interest in view of the still unsettled question of the transformation of fat into carbohydrate. Blixenkrone-M0ller [1938] found an increase of liver glycogen after perfusion of cat liver with Na butyrate, but not with fl-hydroxybutyrate. Haarmann & Schroeder [1938] described the formation of reducing substances from ,B-hydroxybutyric and dihydroxybutyric acids, but no evidence was brought forward by which these substances could be conclusively identified as carbohydrates. How important this identification is will be obvious in the light of the fact that, while the reducing compound formed by rat kidney slices from the first substance is glucose, it is mainly oc-hydroxyacetoacetic acid or acetol in the second case. Benoy & Elliott [1937] showed that a considerable increase of total carbohydrate is found after the aerobic incubation of rat kidney slices with pyruvate and, to a smaller extent, also with lactate, succinate or malate, though no synthesis of glycogen occurs in kidney.

The formation of glucose from acetoacetic acid in rat kidney