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THE following paper presents a preliminary account of the use of ferricyanide as a reagent for the manometric study of dehydrogenase systems, and is intended to form an introduction to more complete studies on this subject. The principle of the new technique depends upon the fact that when 1 mol. ferricyanide is reduced, 1 mol. acid is formed and in bicarbonate media this gives rise to 1 mol. CO2 which can be estimated manometrically. The relevant equations are:

Anaerobic oxidations. On ferricyanide as a reagent for the manometric investigation of dehydrogenase systems