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PART I. FOR several vitamins the quantity necessary to maintain health depends on the composition of the diet. Rats, for instance, need more vitamin D while on a diet with little phosphorus and with abundant calcium than with P and Ca offered in equal doses. It is known also that man requires more vitamin B1 the greater the carbohydrate content of the food. As to vitamin C, Ahmad [1936] and Chakraborty & Roy [1936] have recently shown that the reducing capacity of urine (against dichlorophenolindophenol) increases during a high protein diet. If there really exists a greater urinary excretion of ascorbic acid during a high protein diet, greater requirements of the organism, too, may be expected. Both authors titrated the urine without previous treatment; they did not eliminate interfering reducing substances other than ascorbic acid by precipitation with mercuric acetate [Emmerie, 1934]. Van Eekelen [1934; 1935, 1] observed a normal amount of ascorbic acid in urine with a very high total reducing capacity (as determined by direct titration), the latter being chiefly due to an increased excretion of thiQsulphate. By evaluating during different diets the amounts of total reducing substances and also of ascorbic acid, conclusions have been reached which differ from those of Ahmad and Chakraborty & Roy.

I. On the relation between diet and urinary output of thiosulphate (and ascorbic acid). II. Human requirements for vitamin C