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INTRODUCTION. IN Part I [Roughton, 1935] data have been given for: (A) The relation between the heat liberated and the percentage saturation of haemoglobin with oxygen when haemoglobin is equilibrated with insufficient oxygen to saturate it completely. (B) The effect of laking and of purification on the heat of reaction of oxygen with haemoglobin. (C) The relation between the heat of the reaction and the pH and buffer content of the solution. This question has also been treated theoretically. (D) Furthermore Bateman & Roughton [1935] have shown that the "slow" heat of reaction between 02 and Hb (as measured by the classical methods of calorimetry) agrees within experimental error with the "rapid" heat liberated in 0-008-0-02 sec. These results all conform to simple expectations. The present paper deals with the last item of the programme, sketched in Part I, namely: (E) The comparison, for purified haemoglobin solutions, of the heat of the reaction, as measured directly, with that calculated indirectly from the effect of temperature on the oxyhaemoglobin dissociation curve by means of the Van't Hoff Isochore, which runs [Adair, quoted by Barcroft, 1928]

The thermochemistry of the oxygen-haemoglobin reaction