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SiNCE Scherer's isolation of inositol [1850], the attempts to devise satisfactory methods for its determination in animal tissues have been many; but the properties of the substance and the absence until recently of any chemical means of its estimation have rendered the problem difficult. The obstacles encountered necessitate the preliminary isolation of the inositol, and most workers have been obliged to follow this by weighing the isolated product. Young [1934, 1] modified the Fleury-Marque technique for the determination of polyhydroxy-compounds, making it suitable for the estimation of 1-5 mg. of inositol in pure solution with considerable accuracy; a development which made possible for the first time a quantitative study of the various isolation procedures and enabled a method to be produced giving 90% recovery of inositol from tissues [Young, 1934, 2]. This probably represents the highest accuracy to be expected from any method on the same general lines; but in common with all other existing procedures it possesses certain features that render it unsuitable for use in any but the simplest experiments on tissues, especially where only small amounts of material are available. At the outset of investigations into post mortem and other changes occurring in the inositol content of animal tissues, it became evident that little headway would be be made until some trustworthy method could be found of determining the inositol contents of amounts of tissues of the order of 5-10 g., and employing also some method of extraction suitable for use in such experiments as the above. The work to be described represents an attempt to meet this need by a modification of the isolation process described by Young [1934, 2] combined with a micro-modification of the same author's method for the determination of inositol in pure solution. An amount of tissue containing not less than 5 mg. of inositol is used (about 3-5 g. of mammalian heart muscle) and the recovery by the process is about 90 %. Extraction of inositol. This has been accomplished either by the extraction of the minced tissue with aqueous acetone [Momose, 1916; Needham, 1923, 1, 2; Young, 1934, 2] or by solution of the tissue in alkali, followed by treatment with precipitating agents such as lead acetate [Rosenberger, 1908; Winter, 1934] to remove contaminating substances. Young [1934, 2] also found that treatment with alkali could be made the first stage of a method of tdetermining inositol in tissues. The use of acetone is inadvisable for two reasons. When small amounts of tissue are to be dealt with, the manipulative difficulties become great, for the

A modification of Young's method for the determination of inositol in animal tissues