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IT was recently shown by Stedman, Stedman and Easson [1932] that, whereas liver esterase and pancreatic lipase are without appreciable action on esters of choline, there is present in the blood-serum of the horse an enzyme which readily hydrolyses such esters, but which exerts little, if any, action on simple esters such as methyl butyrate. In order to emphasise the specific nature of its activity, the new enzyme was termed choline-esterase. No other sera were examined at the time for the presence of this enzyme owing to the difficulty in measuring its activity in horse-serum with any degree of accuracy without first submitting it to extensive purification. Further experience with the work has, however, now enabled us so to modify the method of estimation that the activity of the choline-esterase present in 1 cc. of serum can be measured with a fair degree of accuracy. It therefore appeared to us to be of interest to examine the bloodsera from a number of species for the presence of this enzyme and, at the same time, to determine the activities of the same sera towards methyl butyrate and tributyrin. Methods of estimation.

A comparison of the choline-esterase activities of the blood-sera from various species