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Rotational dynamics at the molecular level could provide additional data regarding protein diffusion and cytoskeleton formation at the cellular level. Due to the isotropic emission pattern of fluorescence molecules, it is challenging to extract rotational information from them during imaging. Metal nanoparticles show a polarization-dependent response and could be used for sensing rotational motion. Nanoparticles as an orientation sensing probe offer bio-compatibility and robustness against photo-blinking and photo-bleaching compared to conventional fluorescent molecules. Previously, asymmetric geometrical structures such as nanorods have been used for orientational imaging. Here, we show orientational imaging of symmetric geometrical structures such as 100 nm isolated silver nanocubes by coupling a hyperspectral detector and a focused ion beam (FIB)-fabricated correlating substrate. More than 100 nanocubes are analyzed to confirm spectral shifts in the scattering spectra due to variations in the orientation of the nanocubes with respect to the incoming light. Results are further validated using finite-difference time-domain simulations. Our observations suggest a novel strategy for high-throughput orientation imaging of nanoparticles.

Hyperspectral dark field optical microscopy for orientational imaging of a single plasmonic nanocube using a physics-based learning method