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The  Z – E  photoisomerization quantum yield of the HBDI −  chromophore is a result of early, non-statistical dynamics around a less reactive I-twisted intersection and later, statistical behavior around the more reactive, near-enantiomeric counterpart.

Internal conversion of the anionic GFP chromophore: in and out of the I-twisted S1/S0 conical intersection seam

Internal conversion of the anionic GFP chromophore: in and out of the I-twisted S₁/S₀ conical intersection seam