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Via  directed evolution of the tRNA synthetase, genetic encoding of a unique DBTD derived ncAA into proteins is realized. The DBTD residue is capable of transducing photon energy into ring-strain energy  in situ  for photo-clicking with diarylsydnone.

chemical science

Expanding the functionality of proteins with genetically encoded dibenzo[<i>b</i>,<i>f</i>][1,4,5]thiadiazepine: a photo-transducer for photo-click decoration

Expanding the functionality of proteins with genetically encoded dibenzo[b,f][1,4,5]thiadiazepine: a photo-transducer for photo-click decoration