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SFG spectra analyzed by the developed Hamiltonian method show that adsorbed BSA molecules on silicone oil are dimers. On treatment with dithiothreitol, some BSA dimers dissociate, resulting in 60% dimer and 40% monomer on the silicone oil surface.

Probing protein aggregation at buried interfaces: distinguishing between adsorbed protein monomers, dimers, and a monomer–dimer mixture in situ