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A total of 18 metabolites were identified in rat, and reference standards of metabolites (M2–M4) were further obtained by biotransformation of ingenol. Hydroxylation, oxygenation, sulfonation, and glucuronidation were major metabolic pathways.

Systematic characterization of metabolic profiles of ingenol in rats by UPLC-Q/TOF-MS and NMR in combination with microbial biotransformation