English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We have exemplified a pretargeted approach to interrogate hypoxia in live cells using radioactive bioorthogonal inverse electron demand Diels-Alder (IEDDA) "click" chemistry. Our novel 18 F-tetrazine probe ([ 18 F]FB-Tz) and 2-nitroimidazole-based TCO targeting molecule ( 8 ) showed statistically significant ( P &lt; 0.0001) uptake in hypoxic cells ( ca. 90 %ID per mg) vs. normoxic cells (&lt;10 %ID per mg) in a 60 min incubation of [ 18 F]FB-Tz. This is the first time that an intracellularly targeted small-molecule for IEDDA "click" has been used in conjunction with a radioactive reporter molecule in live cells and may be a useful tool with far-reaching applicability for a variety of applications.

Detecting hypoxia in vitro using 18F-pretargeted IEDDA “click” chemistry in live cells

Detecting hypoxia in vitro using ¹⁸F-pretargeted IEDDA “click” chemistry in live cells