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A covalently bound hybrid between an atomically precise gold nanocluster and a fluorescent dye is loaded into cells  via  endocytosis. Acidification of the endosomes activates fluorescence, enabling optical pH sensing inside live cells.

Covalent and non-covalent coupling of a Au102 nanocluster with a fluorophore: energy transfer, quenching and intracellular pH sensing

Covalent and non-covalent coupling of a Au₁₀₂ nanocluster with a fluorophore: energy transfer, quenching and intracellular pH sensing