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G-quadruplex (G4) DNA inhibits RNA unwinding activity but promotes liquid–liquid phase separation of the DEAD-box helicase Ded1p  in vitro  and in cells. This highlights multifaceted effects of G4DNA on an enzyme with intrinsically disordered domains.

G-quadruplex DNA inhibits unwinding activity but promotes liquid–liquid phase separation by the DEAD-box helicase Ded1p