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A new convenient method of ureido linkage formation in  t6A / ms2t6A  nucleosides greatly enhances the availability of phosphoramidite monomers for the chemical synthesis of modified tRNA fragments.

rsc advances

Efficient access to 3′-O-phosphoramidite derivatives of tRNA relatedN6-threonylcarbamoyladenosine (t6A) and 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A)