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2-Hydroxy-4 H -quinolizin-4-one scaffolds were enzymatically synthesized by integrating three enzymes including phenylacetate-CoA ligase (PcPCL) from an endophytic fungus Penicillium chrysogenum MT-12, malonyl-CoA synthase (AtMatB) from Arabidopsis thaliana , and a type III polyketide synthase (HsPKS3) from Chinese club moss Huperzia serrata . The findings paved the way to produce these kinds of structurally interesting alkaloids by engineered microorganisms.

rsc advances

Enzymatic synthesis of 2-hydroxy-4<i>H</i>-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from <i>Huperzia serrata</i>

Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata