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3′- O -(2-Thio-1,3,2-oxathiaphospholane) derivatives of 5′- O -DMT-LNA-nucleosides were used to prepare P-stereodefined ( R  P -PS)-DNA#LNA chimeras, which form thermally stable parallel complexes with (2′-OMe)-RNA matrices.

rsc advances

LNA units present in [<i>R</i><sub>P</sub>-PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2′-OMe)-RNA strands

LNA units present in [RP-PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2′-OMe)-RNA strands

LNA units present in [R_P-PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2′-OMe)-RNA strands