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The nonspecific uptake of aptamers by dead cells is an often-overlooked factor during the study of aptamer binding to their targets in vivo . Accounting for this can aid in the identification of aptamers with high affinity and specificity.

analytical methods

Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers