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The synthesis of carbon dots (CDs) with long wavelengths, particularly the red-emitting ones, has always been the focus of researchers, and a carbon source is critical in this process. In this study, we report the synthesis of red-emitting CDs (CD-tetra) via a one-step solvothermal method with 1,2,4,5-benzenetetramine tetrahydrochloride as a novel carbon source and ethanol as a solvent, and the quantum yield (QY) of CDs is as high as 30.2%. Middle chromatography isolated gel (MCI Gel) column was used to obtain R-CDs, O-CDs and Y-CDs with emission wavelengths at 619, 608 and 554 nm, respectively. It was discovered that these CDs exhibited great differences in their particle sizes and elemental compositions. Moreover, the fluorescence of the CD-tetra could be efficiently quenched using methylene blue (MB). Under optimal conditions, a linear relationship between the decreased fluorescence intensity of the CD-tetra and the concentration of MB was established in the range of 0.05-9.5 μM. The limit of detection (LOD) is 10 nM, suggesting a promising assay for the detection of MB.

One-step synthesis of red-emitting carbon dots via a solvothermal method and its application in the detection of methylene blue