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Nowadays quantitative chemical analysis is usually costly, instrument-dependent, and time-consuming, which limits its implementation for remote locations and resource-limited regions. Inspired by the ancient papercutting art ( kirigami ), we herein introduce a novel cut-and-paste protocol to fabricate 3D microfluidic paper-based analytical devices (μPADs) that are suitable for on-site quantitative assay applications. The preparation of the device is fast, simple, and independent of any lithographic devices or masks. Particularly designed reaction "channels" were pre-cut from a piece of filter paper, then assembled back to the silanized, superhydrophobic paper pads. The different layers of the device were assembled using a chemically-inert adhesive spray. The fabricated device has high efficiency of liquid handling (up to 60 times faster than conventional methods) and it is particularly inexpensive. Beyond the benchtop fabrication advantage, in conjunction with a custom mobile app developed for colorimetric analysis, we were able to quantify representative environmental contaminants ( i.e. , the amount of Cr(vi) and nitrite ions) in various water samples with the cut-and-paste μPADs (namely kPADs). Their detection limits (0.7 μg mL -1 for Cr(vi) and 0.4 μg mL -1 for nitrite ions, respectively) are comparable with conventional spectrophotometric methods, which confirm the potential of kPADs for on-site environmental/sanitary monitoring and food toxin pre-screening.

From kirigami to three-dimensional paper-based micro-analytical device: cut-and-paste fabrication and mobile app quantitation